PHYTOCHEMICAL AND ANTIOXIDANT PROPERTIES OF MANILKARA ZAPOTA (L.) P ROYEN FRUIT EXTRACTS AND ITS FORMULATION FOR COSMECEUTICAL APPLICATION
Ziti Akhtar Shafii*, Mahiran Basri, Emilia Abdul Malek and Maznah Ismail
ABSTRACT
Manilkara zapota (L.) P fruit extracts were investigated for its bioactive compounds and their potential of natural antioxidant properties for cosmeceutical application. In vitro antioxidant activity of hexane, ethyl acetate, and ethanol extracts were evaluated by 1,1-diphenyl- picrylhydrazyl (DPPH) radical scavenging activity, ß-carotene bleaching activity assays (BCB) and oxygen radical absorbance capacity (ORAC) methods. Total phenolic (TPC), total flavonoid (TFC) and total tannin (TT) of the extracts were determined. Antioxidant activity by DPPH scavenging activity has a good correlation with phenolic and flavonoid content R2= 0.9905 and R2= 0.9924. Whereas, no correlation was shown between ORAC and TPC values (R2 < 0.1). Liquid chromatography mass spectrometry (LCMS) was used to analyze the amount of targeted phenolic and fatty acids in EtPE, EaPE and ethyl acetate seed extract (EaSE). EaPE and EtPE extracts demonstrated strong antioxidant activity than HSE (3.09±0.08 μg/mL, 2.57±0.18 μg/mL and 7.54±0.17 μg/mL (p<0.05), respectively). However, EtPE extract was chosen to be formulated and studied as ethanol is much cheaper than ethyl acetate as solvent for extraction. The formulation exhibited non-toxic property and thus safe to be explored in cosmeceutical application.
Keywords: Antioxidant activity, phytochemical, Manilkara zapota, cytotoxicity.
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