European Journal of Biomedical and Pharmaceutical Sciences

PHYTOCHEMICAL ANALYSIS OF NEEM LEAVES EXTRACT THROUGH GC-MS IN METHANOL SOLVENT AND ANTIFUNGAL ACTIVITIES OF NEEM LEAVES EXTRACT AGAINST DIFFERENT FUNGAL PATHOGENS

Pandey A. K.*, Sajad Ahmad Mir, Abid Hussain Qureshi and Basharat Suhail

ABSTRACT

GC-MS chromatogram of methanolic extract of Azadirachta indica showed five major peaks. From the result of GC-MS it shows the presence of phytol, linolenic acid, α linolenic acid, γ linolenic acid, palmitic acid and Tridecanolic acid. 1 propanol was the major componet in the extract. Extract in methanol solvents were found to be effective to inhibition against different fungal pathogens viz, Fusarium oxysporum, Aspergillus flavus, Aspergillus niger, Aspergillus fumigatus, Penicillium and Alternaria alternata species. There was no zone of inhibition in Rhizopus stolonifer at any concentration. Zone of inhibition was seen lowest at 25 μl concentrations while at 50 μl and 75 μl concentration zone of inhibition shows progressive change in which, Fusarium oxysporium, Aspergillus flavus and A. niger shows zone of inhibition in the range of 14.92 mm at 50 μl and 15.62mm at 75 μl in Aspergillus flavus. 16.72mm at 50 μl and 21.09mm at 75 μl concentration in Fusarium oxysporium, 9.62mm in Alternaria alternata at 50 μl and 12.24mm at 75 μl concentration.

Keywords: GC-MS analysis, Azadirachta indica, Antifungal activity, Methanol solvent.