European Journal of Biomedical and Pharmaceutical Sciences

NEW SPECTROPHOTOMETRIC ESTIMATION OF METRONIDAZOLE IN THE TABLETS USING MIXED SOLVENCY APPROACH

Sanjay Jain*, R. K. Maheshwari, Rajesh Kumar Nema and Indrajeet Singhvi

ABSTRACT

Organic solvents are most frequently employed in spectrophotometric analyses. They may be sources of pollution. Some of them may be toxic while others may be costlier. Volatility may be a source of inaccuracy in spectrophotometric estimations. In the present investigation, it was proposed to solubilize Metronidazole by use of mixed solvency concept. Metronidazole shows maximum absorbance in the concentration range of 5-25 μg/ml at 320 nm. Method of analyses have been validated for different parameters like linearity, accuracy, precision, LOD and LOQ. The percent drug estimated in tablet formulation of Batch-I and of Batch-II were 99.74±0.735 and 99.97±0.784 respectively. The range of percent recoveries varied from 99.95±0.242 to 101.32±0.596. The analytical method was found to be simple, safe (free from toxicity), economic and eco-friendly.

Keywords: Metronidazole, UV-Spectrophotometry, solid dosage formulation, mixed solvency concept.