Abstract
GREEN SYNTHESIS OF NANOPARTICLES FROM ETHANOL EXTRACT OF COFFEA ARABICA UNPROCESSED BEANS AND EVALUATING ITS ANTI-BACTERIAL ACTIVITY

Jamila Rampurawala, Vedamurthy A.B., Padmaa M Paarakh* and Sudisha Jogaiah

ABSTRACT

The biosynthesis of nanoparticles has been proposed as a cost effective and environmental friendly alternative to chemical and physical methods. Plant mediated synthesis of nanoparticles is a green chemistry approach that interconnects nanotechnology and plant biotechnology. Coffea arabica is most common species of coffee found and consumed in India and has great economic importance. In the present study, green synthesis of 7 nanoparticles has been demonstrated using ethanol extract of unprocessed [unbaked] beans of Coffea arabica reducing silver, ferrous, ferric, magnesium, manganese, lead and copper ions. The study was also carried out to evaluate antibacterial activity of 7 nanoparticles against Staphylococcus aureus, Escherichia coli, Shigella dysenteriae, Klebsiella pneumonia and Bacillus subtilis using agar well diffusion method. The study exhibited that ethanol extract of unprocessed beans of Coffea arabica produced 7 nanoparticles. In the antibacterial activity, the inhibition zone for Staphylococcus aureus, Escherichia coli, Shigella dysenteriae, Klebsiella pneumonia and Bacillus subtilis were determined. Out of 7 nanoparticles, silver, copper and ferrous nanoparticles showed better activity against microorganism used. This synthesis approach of 7 nanoparticles is cost effective and can be widely used in biological systems. This experiment has concluded the strong antibacterial properties of nanoparticles prepared by unprocessed beans of C. arabica. Silver, copper and ferrous nanoparticles proved to be the most potent amongst all the nanoparticles synthesized as they inhibited maximum type of bacteria under test. These nanoparticles stand as promising candidates for use as antibacterials and further studies are needed to substantiate the claims.

Keywords: Coffea arabica, ethanol extract,Nanoparticles, AgNPs, CuNPs, Fe2+NPs, Fe3+NPs, MgNPs, PbNPs, MnNPs, Antibacterial activity.


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