ANTICANCER EFFICACY OF GELATINE-ETOPOSIDE NANOPARTICLES IN NCI-H460 CELL LINE
Lakhya Jyoti Gogoi* and Kamala Kanta Borah
ABSTRACT
Chemotherapy has been a major treatment methodology for malignant tumors. Etoposide (ETP), as one of the oldest and best antieoplastic chemotherapy drugs. However, ETP has some drawbacks such as unstabilization, poor water solubility and short biological half time, which limit the uses of ETP in medicine. The aim of the present study was to encapsulate ETP in gelatin nanoparticles (GNPs) and to study its anticancer efficacy in NCI-H460 non small cell lung carcinoma cell line. SEM and DLS studies have revealed that the prepared ETP-GNPs possess spherical shape with a mean diameter of 186 nm. Through the Schiff base reaction and hydrogen bond interaction of cross-linker glutaraldehyde has been achieved the successful encapsulation of ETP in GNPs. In vitro drug release kinetics indicated that there was an initial burst release followed by a slow and sustained release of ETP from GNPs. Further, ETP-GNPs exhibits increased intracellular ROS levels and apoptotic morphological changes in NCI-H460 cells when compared to bulk ETP treatment alone. Hence, GNPs carrier system might be a promising mode for controlled delivery and for improved therapeutic index of poorly water soluble ETP.
Keywords: Chemotherapy, Nanoparticle, Anticancer, Etoposide (ETP), Gelatin Nanoparticles (GNPs).
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