CHROMATOGRAPHIC ANALYSIS OF RHIZOPHORA MUCRONATA BARK- BASED ON ANTIOXIDANT AND FREE RADICAL SCAVENGING POTENTIAL
Chitra Jairaman, Syed Ali Mohammed Yacoob*, Anuradha Venkataraman, Yogananth Nagarajan and Asrar Mohamed
ABSTRACT
The present study aims at performing the total anti oxidant capacity, total phenolics, total flavonoids, free radical scavenging which includes DPPH Assay, H2O2 scavenging assay, SO radical scavenging of the different bark extract of Rhizophora mucronata and characterization of the components present in it by HPTLC fingerprinting. The various solvent extracts of Rhizophora mucronata bark were subjected to anti oxidant assay and free radical scavenging assay. Total Phenolic content ranged from 22.17 to 85.4 mg/gm of gallic acid equivalent, Total Flavonoid from 21.27 to 84.72 mg/gm Quercetin equivalent and Total antioxidant capacity was recorded between 29 to 78.5 mg/gm Ascorbic acid equivalent. The DPPH assay has been found to be maximum for ethanol: water (7:3) showed 94.56% inhibition and least recorded for diethyl ether with 41.45% inhibition. Similar results were obtained for other scavenging assay viz SO and H2O2 radical scavenging assay. The preliminary phytochemical analysis of these extracts revealed the presence of alkaloids, terpenoids, phenols, phyto sterols, carbohydrates, flavonoids, saponins, tannins. Based on the phytochemical analysis, ethanol: water (7:3) was selected as the most suitable extract for further analysis as it has eluded 8 major components. Thus, ethanol: water (7:3) was then subjected to column chromatography to yield different fractions. The HPTLC results obtained clearly indicated the flavonoid: Quercetin is found both in crude and fractions BE-3 and BE-4 as well. Thus, it can be concluded that the bark of Rhizophora contains powerful bioactive compounds with huge therapeutic activity.
Keywords: Rhizophora mucronata, Phytoconstituents, Anti oxidant capacity, DPPH Assay, HPTLC Fingerprinting.
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