European Journal of Biomedical and Pharmaceutical Sciences

BIOSYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM MURRAYA KOENIGII

V. Ramamurthy* and A. S. Prasanth

ABSTRACT

The nanoparticle is a novel way to synthesis nanoparticles by using biological sources. It is gaining attention due to its cost effective, ecofriendly and large scale production possibilities. In this present study Murraya koenigii was taken to investigate their potential for synthesizing silver nanoparticle. The silver nanoparticles synthesized were confirmed by its change of colour to dark brown due to the phenomenon of surface plasmon resonance. Ag NPs were characterized by UV–vis spectrophotometer, SEM, XRD, AFM and FTIR spectroscopy. Murraya koenigii leaf showed great capability to synthesis Ag NPs at optimum temperature conditions. The UV absorption peak at 428nm clearly indicates the synthesis of Ag NPs. The SEM, TEM and AFM studies were helpful at deciphering their morphology and distribution. DLS and Zeta potential studies validated the size and charge of the nanoparticles in the colloidal system without any aggregation. FTIR studies confirmed the biofabrication of the Ag NPs by the action of different phytochemicals with its different functional groups present in the extract solution. The XRD patterns confirmed the purity, phase composition and nature of the synthesized nanoparticles. The silver nanoparticles have great pharmacological activity.

Keywords: Green synthesis, Silver nanoparticles, Murraya koenigii.