Abstract
HPTLC FINGERPRINTINGS OF ETHYL ACETATE EXTRACT OF CYNOGLOSSUM ZEYLANICUM (BORAGINACEAE)

Balan P*, Ansiyasiraj, Balasubramanaian T and Suriyaprakash TNK

ABSTRACT

Background: Qualitative and Quantitative assessment is a fundamental requirement for standatization of herbal drugs. High performance thin layer chromatography (HPTLC) is the best method for the screening of herbal drugs with respect to different secondary metabolite with their quality and quantity. Aim: To study the HPTLC fingerprinting profile of ethyl acetate extract of Cynoglossum zeylanicum (Boraginaceae). Methods: A CAMAG HPTLC system equipped with LINOMAT 5 applicator, a TLC scanner 3, REPROSTAR 3 and WIN CATS-4 software were used. Results: The study revealed the presence of coumarin glycosides (12 peaks), terpenoids (11 peaks) and steroids (6 peaks) in ethyl acetate extract of Cynoglossum zeylanicum Conclusion: The HPTLC fingerprinting profile developed for ethyl acetate extract would help in proper identification and quantification of lead compounds. By isolating and identifying lead compounds, new lead molecule can be isolated to treat the diseases.

Keywords: HPTLC; Ethyl acetate; Cynoglossum zeylanicum; Coumarin glycosides; Terpenoids; Steroids.


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