THE EFFECT OF COMPETITIVE AND NONCOMPETIVE ANTAGONIST OF ASPARTATE ON THE SURVIVAL TIME OF MICE WITH EXPERIMENTAL TETANUS
Mujezinovic Indira, Smajlovic Ahmed, Katica Muhamed*, Gradascevic Nedzad and Dukic Behija
ABSTRACT
Tetanus is dangerous, infectious, noncontagious, acute and mostly afebrile illness of humans and different animal species, which is caused by Gram positive, ubiqutarious anaerobic Clostridium tetani. It produces a specific neurotoxin, which consists of two components: tetanospasmin and tetanolysin. Tetanospasmin is a component of tetanus toxin which actually leads to emergence of clinical signs. Mechanism of action of tetanus toxin is still unknown, but it is clear that the main target site is the spinal cord, where it blocks the release of inhibitory transmitters which results in domination of excitatory transmitters. In our experiment, we tried to normalize the disorders which are results of tetanus toxin action by applying indol-2-carboxylic acid (at doses of 5, 10, 20, 30 and 50 mg/kg b.w.) and to investigate its combination with ketamine (at dose of 10 mg/kg b.w.) and aminooxyacetic acid (at dose of 20 mg/kg b.w). Experiments were conducted on albino mice of both sexes. Experimental tetanus was induced by intramuscular application of tetanus toxin at a dose of 0.2 μg per animal. Application of substances in experimental groups was performed after the occurrence of local tetanus in right leg, approximately 24 hours after administration of tetanus toxin. Through our research, we found that indol-2-carboxylic acid had the best effect at a dose of 20 mg/kg b.w. Additionally, combination of indol-2-carboxylic acid with ketamine and aminooxyacetic acid had weak effect on the half time survival period in mice with experimental tetanus in the trial, as compared to the control group.
Keywords: Tetanus toxin, indol-2-carboxylic acid, ketamine, aminooxyacetic acid.
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