Abstract
BIOSYNTHESIS, CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES USING THE LICHEN PARMOTREMA PERLATUM

M. Revathy, A.Mathiazhagan and G. Annadurai*

ABSTRACT

Currently, Green nanotechnology principle is highly applicable in synthesis of nanoparticles through simple techniques. In this study, we demonstrate the synthesis of silver nanoparticle by ethanol extract of algae fungi intermediate lichen Parmotrema perlatum. The synthesized silver nanoparticles were analyzed by UV visible spectrophotometer, XRD, FTIR, and SEM analysis. Lichen extract could be able to synthesize silver nanoparticles as proved by gradual change in the color of the reaction mixture consists of 10 ml extract in 1mM Agno3 to dark brown color. Silver nanoparticles formation was confirmed by surface Plasmon resonance (SPR) peak obtained around 400 – 420 nm in UV Visible spectrophotometer. The morphology of the biogenic silver nanoparticles was identified by SEM analysis and is polydispersed in nature. The occurrence of silver in the sample and its crystalline nature was confirmed by X-ray diffraction technique. Silver nanoparticles showed 2ϴ values within the range of Bragg’s reflection (38.06 and 77.43) consequent to the existence of silver nanocrystal as obvious by X-ray diffraction spectrum. FTIR results were used to identify the biomolecules which is responsible for the silver ion reduction. The FTIR results of lichen extract synthesized silver nanoparticle displayed the prominent peaks in (3210.17, 2849.93, 2917.38, 1652.85, 1609.87, 1452.63, 1378.45, 1145.25, 823.36, 585.50, 527.27) in different ranges. These biogenic silver nanoparticles exhibited substantial antibacterial efficacy against both Gram positive and Gram negative organisms such as Enterococcus, streptococcus, Escherichia coli, pneumoniae, klebsiella, serratia, planomicrobium. The Invitro antibacterial test 100g disk-1 concentration displayed the results of maximum inhibition zone with the Enterococcus (30 mm) and minimum inhibition zone with klebsiella sp (7mm). The antibacterial activity of silver nanoparticles against these bacterial cultures was evaluated to obtain their promising use in silver containing antibacterial product. Lichen extract has the ability to treat various skin diseases and applying in wound healing treatments. Thus the silver nanoparticles synthesized by lichen extract may have the potential biomedical applications.

Keywords: Parmotrema perlatum, silver nanoparticle, Characterization, antibacterial activity.


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