European Journal of Biomedical and Pharmaceutical Sciences

DAIRY BACTERIOLOGY AND MICROBIAL ANALYSIS OF MILK USING SYRINGE FILTER

*Arulraj Panneerselvam., Asarudheen I., Jasmin T., Mohammed sarfas., Sahnas N. K., Vedha Satheesh., Venkatanarayanan R.

ABSTRACT

Objective: To find the effect of syringe filter for the sterilization of different milk samples. Raw cow milk, commercial Pasteurized milk and goat milk was taken as the samples for the analysis. Method: The sterility testing was done for the three samples with direct inoculation method using pour plate method and spread plate method. The filtration sterilization was performed by using single use syringe filter. Result: Highlighted spread plate method as it gives maximum number of colonies. 437 number of colonies were observed in the goat milk, 184, 326 number of colonies were found in commercial pasteurized milk and raw cow milk respectively. Conclusion: We decided to find the effectiveness of the sterilization of the above milk samples by using syringe filter (minisart single use syringe filter Non-pyrogenic 0.2 micrometer). After sterilization the number of colonies were nil in both commercial pasteurized milk and goat milk but 21 number of colonies were still observed in raw cow milk. Hence concluded that the use of syringe filter shall be for commercial and goat milk but not effective for raw cow milk.

Keywords: Milk analysis, test for sterility, direct inoculation method, Syringe filter.