IN VIVO ANTIOXIDANT, ANTI-INFLAMMATORY AND TLC PROFILING OF INDIAN KINO IN RAT MODEL
Aruna L. Hugar, Amarvani P. Kanjikar and Ramesh L. Londonkar*
ABSTRACT
Background: Polycystic ovary syndrome (PCOS), the most common endocrine and metabolic disorders of reproductive-aged women, is characterized by menstrual disturbance, infertility, oxidative stress and ovary inflammation. Herbal medicines are known to cure these disorders with fewer side effects. Among them, Pterocarpus marsupium, which is commonly known as Indian Kino, has a long history of numerous traditional and ethnobotanical applications in diverse cultures. Objective: To investigate invivo antioxidant and anti-inflammatory activities of P.marsupium along with TLC profiling. Methodology: In vivo antioxidant activity was carried out in PCOS induced female albino rats as described by Mamata et.al (2013). In vivo anti-inflammatory activity was measured by carrageenan induced hind paw oedema assay. Thin layer chromatographic profiling of methanol extract was also conducted to identify the bioactive compounds. Results: Upon administration of low and high dose of Pterocarpus marsupium methanol extract to PCOS induced rats indicated increase in CAT, GSH and SOD enzymes but decreased in MDA tissue content when compared to normal control and PCOS treated group. The extract has also exhibited dose dependent anti-inflammatory activity. TLC profiling of methanol extract confirms the presence of various phytochemicals. Conclusion: The results of methanol extract of P.marsupium indicates significant in vivo antioxidant and anti-inflammatory activities, which can be considered as a promising plant source of natural antioxidant and anti-inflammation that is due to the presence of phytoactive compounds confirmed through TLC.
Keywords: Pterocarpus marsupium, SOD, CAT, carrageenan hind paw oedema, female albino rats, TLC.
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