European Journal of Biomedical and Pharmaceutical Sciences

SCREENING AND CHARACTERIZATION OF ALPHA AMYLASE INHIBITOR PRODUCING ISOLATE- STREPTOMYCES LATERITIUS- SS4/6 USING 16SrDNA TECHNIQUE

Nagamani Bolla*, T. Prabhakar and G. Girija Sankar

ABSTRACT

Alpha amylase inhibitors play an important role in reducing blood glucose levels. Inhibiting α-amylase enzyme plays a crucial part in treatment for insulin-dependent diabetes mellitus, obesity and hyperlipedemia. Amylase inhibitors are useful tools for the determination of activities of amylase isozyme and purification of amylases. Most of the amylase inhibitors were isolated from plants and microorganisms using different screening techniques. Screening of 200 marine microorganisms resulted in isolation of one actinomycete strain with the ability to secrete potent alpha amylase inhibitor was designated as SS4/6 and characterized by using 16s rDNA sequencing and phylogenetic characterization. The isolate SS4/6 was identified as Streptomyces lateritius by IMTECH Chandigarh with an accession number MTCC 10474. The isolate was able to produce potent alpha amylase inhibitor which can inhibit both salivary alpha amylase and pancreatic alpha amylase.

Keywords: Streptomyces lateritius, 16SrDNA, TLC method phylogenetic tree and starch agar plate method.