PRELIMINARY PHYTOCHEMICAL INVESTIGATION AND ESTIMATION OF POLYPHENOLICS IN DIFFERENT PARTS OF SELECTED SPECIES OF JUSTICIA
Sini S., Prashy P. and Satheesh George*
ABSTRACT
The present research work aimed at gaining comparative phytochemical data set of different plant parts of selected Justicia species from Kerala, i.e. J. adhatoda, J. beddomei, J. betonica, J. carnea, J. gendarussa, J. montana, and J. wayanadensis. The ployphenolic content of seven species of Justicia are determined in the present work. The phenolic content was determined using Folin-Ciocalteu assay. Phytochemical investigation was carried out using standardized phytochemical tests. The results show that different parts of selected Justica species contains significant amount of phenolic content. Highest phenolic content was estimated in aqueous extracts of various parts in comparison to that in absolute methanol extracts. Highest phenolic content in aqueous extract was shown by leaf of J. adhatoda (18.67 mg/GAE/g) and lowest for root of J. gendarussa (1.62 mg/GAE/g). In the case of absolute methanolic extracts, highest value of phenolic content was shown by root of J. beddomei (10.11 mg/GAE/g) and least phenolic content was as shown by root of J. carnea (1.69 mg/GAE/g). Different parts of selected Justicia species revealed the presence of alkaloids, phytosterols, phenolic and flavonoid compounds, tannins, proteins, oils, saponins etc. after phytochemical analysis.
Keywords: Justicia, Phytochemical, polyphenolic content, aqueous extract, absolute methanolic extracts.
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