ESSENTIAL OIL COMPOSITION AND ANTIOXIDANT, ANTIBACTERIAL ACTIVITY OF LEAF EXTRACT OF PERSEA ODORATISSIMA (NEES)
Pritee Pant*, Kapil Khulbe and Charu Chandra Pant
ABSTRACT
Present study deals with the phytochemical analysis, antioxidant and antibacterial activity of leaf extract of Persea odoratissima. Beside these GC-MS analysis of volatile oil of leaf was also done. P. odoratissima belongs to the family lauraceae used in the folk medicine as antiseptic and anti-inflammatory remedies. The two solvent methanol and hexane used for the extraction of leaves and different methods used for the exploration of the qualitative phytochemical analysis, antioxidant like total phenolic contain, total flavonoid contain, DPPH, Ferric reducing analysis, and antibacterial test by disc diffusion method. The oil sample was analyzed by GC and GC-MS and the components were identified on the basis of their RI values. Quantitative evaluations of methanol and hexane extract revealed that the presence of effective amount of phenol (88.06mgGAE/g dry weight and 0.64mgGAE/g dry weight respectively) and flavonoid (6.28mgQE/g dry weight and3.08 mgQE/g dry weight) Ferric reducing power of the plant found the 65.63 and 0.84AAE/g dry weight for methanol and hexane extract respectively. Plant showed high antioxidant potential by DPPH method (IC50 7.90μg/ml μg/ml and 118.84μg/ml for methanol and hexane respectively), which was showed close relation with ascorbic acid (IC50 5.48) used as a standard. The extract were tested against various bacterial strain at different concentration the inhibition zones were in the range of 15.38 mm to 6.5 mm in 100 mg/ml to 12.5mg/ml concentration respectively. GC-MS analysis of the leaf oil was found to be rich in Oxigenated sesquiterpenes (50.57%).
Keywords: Antioxidant activity, phytochemical analysis, antibacterial activity, DPPH, GC-MS analysis.
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