Abstract
PHYTOCHEMICAL CHARACTERIZATION OF ALOE BARBANDENSIS MILLER AND ITS ANTIOXIDANT ACTIVITY

Shobhna Tiwari* and Dr. M. K. Gupta

ABSTRACT

The aim of the study was to analyse phytochemical constituents of Aloe barbadensis Miller and its antioxidant activity. The qualitative study was done by using 4 different extracts of Aloe barbadensis which were petroleum ether, chloroform, ethyl acetate and 70% methanolic extract. Of these extracts 70% methanolic extract have shown the best results as it showed the presence of carbohydrates, anthraquinone glycosides, alkaloids, saponins, flavonoids, triterpenes and fats and oils. On the basis of these tests it was decided to take 70% methanolic extract for quantitative analysis and antioxidant assay. For quantitative analysis total phenolic content and total flavonoid content estimation were done and for antioxidant activity DPPH 2,2-diphenyl-1-picryl-hydrazyl-hydrate) assay and reducing power assay were done. The antioxidant activity was determined at the concentration of 20,40,60 80 and 100μg/ml and IC 50 was calculated.

Keywords: Aloe barbadensis, carbohydrates, anthraquinone glycosides.


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