STUDIES ON ESTRADIOL INDUCED APOPTOSIS IN RAT TESTIS
Vyas A.*, Purohit A., Singh M. and Kalla N. R.
ABSTRACT
Estrogens have been reported to inhibit the testis function. The effect is mediated by inhibition of gonadotropin action which in turn inhibits testosterone production. Present investigations were made to evaluate the effect of estradiol on the induction of apoptosis in male germ cells. To rule out the possibility of the inhibitory effect of estradiol on gonadotropin secretion, hypophysectomised gonadotropin stimulated male rats were used. In the present study we have studied the ability of estradiol to induce apoptosis in the male germ cells. The presence of apoptosis was assessed by demonstrating a DNA ladder and by TUNEL technique in situ. It was found that the number of degenerating cells after treatment with 1 E2cm capsule increases with comparison to controls. In order to find out this the parafinised testes sections were subjected to labeling of fragmented DNA, using Oncor Apoptag kit (TUNEL). It was found that the number of apoptotic cells increases 5 folds with 1 cmE2 capsule after 4 days but with 4 cm E2capsule the presence of large number of giant cells contribute much to the loss of germ cells than apoptosis suggesting that 4 cmE2 capsule is too massive a dose for the cells to enter into apoptosis and the reduction in the number of spermatozoa is due to necrosis rather than apoptosis.
Keywords: Apoptosis, Apoptotic Index, Estradiol, Seminiferous tubules, Sialistic Capsule, TUNEL.
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