European Journal of Biomedical and Pharmaceutical Sciences

ACTIVITY OF HUMAN PLASMA PROTEINS ON TRYPANOSOMIASIS

Safaa M. Barghash*, Hassan M. Sobhy, Rasha S. Nono and Esam A. Razin

ABSTRACT

It is the first one had been conducted in Egypt to evaluate the antitrypanosomal activity of human plasma proteins compared with diminazene aceturate (DA) using Albino rats as a biological model. It was also carried out to assess the behavior, hematological, biochemical, and histopathological changes that may be associated with the administration of plasmas of four blood groups (O+, AB+, B+, A+) at a dose of 0.5 ml/200 gm. b. wt., two times along the experiment, for a period of 42 days against Trypanosoma evansi infection. The in vitro test for antitrypanosomal activity revealed that all the tested plasmas cause the disappearance of the Trypanosoma parasite at a very low concentration (0.001%). Results also revealed that treated groups with the plasma proteins of O+ and B+ human blood groups exhibited the highest in vitro and in vivo antitrypanosomal effect against T. evansi followed by A+ and AB+ groups, and could be useful in the management of trypanosomiasis. Histopathological examination evidenced and confirmed that O+ treated animals followed by B+ did not suffer from abnormalities in contrast to A+ and AB+ treated animals.

Keywords: Trypanosoma evansi, Plasma proteins, Hematology, Biochemical analysis, Histopathology, Egypt.