European Journal of Biomedical and Pharmaceutical Sciences

PSEUDOMONAS AERUGINOSA PIGMENT- BIO COLOURS AS MOST POTENT ANTIBACTERIAL AGENT AGAINST S.AUREUS ISOLATE FROM CURRENCY NOTES

Achiffa Abdul Rahiman*, Aeliya Zehra, M. Ayisha Begum, S. K. Aishwarya, R. Ushasri

ABSTRACT

Pseudomonas aeruginosa common pathogenic bacteria belonging to family pseudomonadaceae is a gram negative, rod shaped bacterium that causes diseases in humans as well as animals and mostly pathogenic to plants. P.aeruginosa is found in terrestrial and aquatic environment especially in moist environment of hospitals resulting in Hospital Acquired Infections. The main aim of the current study was to determine the antibacterial activity of crude acetone pigment extract of Pseudomonas aeruginosa against Staphylococcus aurous isolated from currency notes. The currency note was collected from the petty shop of a crowded area in a sterile zip lock cover. It was then transferred to Nutrient broth for the processing of the sample. The sample was observed under microscopy and processed by inoculating into the nutrient broth and incubated for 24 hours at 37°C and broth culture was centrifuged followed by inoculation in to various types of media. The isolate was identified as Staphylococcus aureus by morphological, cultural, biochemical characters. The bacterial pigment was extracted by sub culturing Psuedomonas aeruginosa. Pseudomonas aeruginosa was inoculated in to conical flask containing nutrient broth and incubated for 24hrs to 72 hrs in rotory shaker for production of pigment. The UV spectroscopic study was done to estimate the peak value of pigmented broth. The crude acetone pigment extract was prepared and assessed for antibacterial activity. The crude acetone pseudomonas pigment extract exhibited activity against s.aureus isolated from currency note which was found to be greater at 500μl with value of 19mm and minimum at 62.5 μl with value of 15mm.

Keywords: Cetrimide agar, spectrophotometer, currency note.