RP-HPLC AND SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF RESVERATROL AND L-ARGININE IN PHARMACEUTICAL DOSAGE FORM
Bhavika Patel*, Dr. Shailesh V. Luhar and Dr. Sachin B. Narkhede
ABSTRACT
A simple, rapid, economical, precise and accurate RP-HPLC method for simultaneous estimation of Resveratrol and L-Arginine has been developed. A reverse phase high performance liquid chromatoghrapic method was developes for simultaneous estimation of Resveratrol and L-Arginine. The separation was achived by LC-2010 CHT C18 (250 cm * 0.46 cm) Shiseido column and Buffer(pH 6.0):Methanol (40:60)as mobie phase , at flow rate of 1 ml/min. Detection was carried out at 230 nm. Retention time of L-Arginine and Resveratrol were found to be 3.143 min and 5.907 min, respectively. The method has been validated for linearity, accuracy and precision. Linearity observed for L-Arginine 5-15 μg/ml and for Resveratrol 6.25-18.75μg/ml. The percentage recoveries obtained for L-Arginine and Resveratrol were found to be in range of 100.181%-99.843% and 99.020%-98.858% respectively. Developed method was found to be accurate, precise and rapid for simultaneous estimation of L-Arginine and Resveratrol. The proposed method was successfully applied for the simultaneous estimation of both the drugs in commercial Combined dosage form.
Keywords: L-Arginine, Resveratrol, Simultaneous estimation, RP-HPLC Method, Validation.
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