European Journal of Biomedical and Pharmaceutical Sciences

STANDARDISATION OF QUALITY PARAMETER AND QUANTIFICATION OF 6-SHOGOAL IN CHATURBHADRA KVATHA CHURNA - A POLYHERBAL AYURVEDIC FORMULATION

U. Prakash Kumar, Indira Balachandran & A. B. Rema Shree*

ABSTRACT

Ayurvedic ‘finished’ medicines belong to diverse classes or categories e.g. churna, kvatha churna, gulika (pills), kashayam, lehyam, gulam, tailam, ghritam, lepa, kuzhambu, asavam, arishtam (besides bhasma, sindoora containing minerals and metals) etc. Standardized ayurvedic formulations of uniform quality are essential for beneficial therapeutic use. As the global market for herbal medicinal product is increasing tremendously, need for quality control parameters which are accepted globally, is being felt. Therefore an attempt has been made in developing standardization parameters covering physico-chemical parameters and TLC/HPTLC/GC profile for the ayurvedic compound formulation Chaturbhadra kvatha churna. Three batches of churnas were prepared; physico chemical parameters and extractions were carried out. The HPTLC analysis was performed on pre-coated silica gel plate 60F-254 plate using n-butanol: acetic acid: water (5: 2: 2) as mobile phase in a CAMAG chamber. Camag TLC Scanner 3 was used for the densitometric scanning at 550nm. Specific marker compounds were used for the quantification. GC was carried out in Agilent GC equipped with HP-5 (5 percent phenyl methyl siloxane) capillary column (30 m x 320 μm x 0.25 μm) with gradient temperature programme. Tests for aflatoxins G1, G2, B1 and B2 were done according to standard procedure. The development of pharmacopoeial standards of Chaturbhadra kvatha churna was based on the outcome of physicochemical, TLC, HPTLC, GC finger print profiles and aflatoxin tests. As a quantitative marker for the churna, 6- Shogaol and 8-gingerol were tried in the chloroform extract (non-sequential) of the churna, using mobile phase hexane: acetone (7:3). Amount of 6-Shogaol present in the churna samples was quantified and it was found to be 0.007, 0.008, 0.0075% with respect to churna. Spectrum of 6-Shogaol and corresponding spot in churna samples were compared. HPTLC profiles of three different batches of CBK churna were compared. The results were found to be highly accurate, quick and reliable for routine monitoring in compound preparations. With the growing demand of herbal drugs market, it is suggested that this standardization tool will help in maintaining the quality and batch to batch consistency of many ayurvedic raw drug powder based preparations.

Keywords: Polyherbal ayurvedic formulation, chaturbhadra kvatha churna, HPTLC, GC, 6-shogoal.