European Journal of Biomedical and Pharmaceutical Sciences

RP-HPLC METHOD DEVELOPMENT AND VALIDATION OFSIMULTANEOUS ESTIMATION OF BETAMETHASONE, GENTAMICIN AND MICONAZOLE IN CREAM FORMULATION

*Meruva Sathish Kumar, B. Ashish Kumar, G. L. Priyanka, K. Hema, M. Prescilla and S. Harshitha

ABSTRACT

A simple, Accurate, precise method was developed for the simultaneous estimation of the Betamethasone, Gentamicin and Miconazole in liquid dosage form. Chromatogram was run through Kromosil 250 x 4.6 mm, 5. Mobile phase containing Buffer and Acetonitrie in the ratio of 50:50 A was pumped through column at a flow rate of 1ml/min. Buffer used in this method was 0.1% OPA buffer at pH 2.5. Temperature was maintained at 30°C. Optimized wavelength for Betamethasone, Gentamicin and Miconazole was 240 nm. Retention time of Betamethasone, Gentamicin and Miconazole were found to be 2.348min, 3.435 min and 4.148 min %RSD of system precision for Betamethasone, Gentamicin and Miconazole. were and found to be 0.3, 0.9 and 0.2 respectively. %RSD of method precision for Chlorthalidone, Gentamicin and Miconazole. were and found to be 0.3, 0.4 and 0.2 respectively. % recovery was Obtained as 99.35%, 98.53%, 99.59% for Betamethasone, Gentamicin and Miconazole. Respectively. LOD, LOQ values are obtained from regression equations of Betamethasone, Gentamicin and Miconazole were 0.06ppm, 0.18ppm, 0.05ppm, 0.15ppm and 0.08ppm, 0.24ppm respectively. Regression equation of Betamethasone. was y =11837.x + 376.1, Gentamicine was y = 12013x +352.8 and of Miconazole was y = 4931.x + 840.9.

Keywords: Betamethasone. Gentamicin. and Miconazole, RP-HPLC.