European Journal of Biomedical and Pharmaceutical Sciences

ANALYTICAL METHOD DEVELOPMENT & VALIDATION FOR CLINDAMYCIN, CLOTRIMAZOLE & TINIDAZOLE IN PHARMACEUTICAL DOSAGE FORM

Priyanka C. Parekh*, Dr. Shailesh V. Luhar and Dr. Sachin B. Narkhede

ABSTRACT

Method A: RP-HPLC Method was studied by using Shimadzu 2010 CHT, Chromatographic separation was achieved using Shiseido C18 RP column (250 mm × 4.6 mm i.d., 5μm) kept at ambient temperature, using a mobile phase consisting a mixture of Phosphate buffer (pH 3.0): methanol (60:40 v/v) and pH adjusted with 0.5% orthophosphoric acid at a flow rate of 1.0 ml/min. The detection was made at 210 nm. Retention time was 3.02 min, 4.13 min and 6.98 min for Clindamycin, Clotrimazole And Tinidazole. Linear correlation was obtained between peak area and concentration in the range of 5-15 μg/ml for Clindamycin, Clotrimazole And Tinidazole respectively. The %RSD value was less than 2, for intraday and interday precision. Method B: Simultaneous Equation Method for Clindamycin Clotrimazole and Tinidazole was studied by UV-3000+, LAB-INDIA. HCl was used as a solvent. The wavelength selected was 216 nm, 230 nm and 296 nm for Clindamycin, Clotrimazole And Tinidazole respectively. Beer’s law is obeyed in the concentration range of 20-100 μg/ml for Clindamycin, Clotrimazole And Tinidazole respectively.

Keywords: Clindamycin, Clotrimazole, Tinidazole, RP-HPLC, UV- Spectroscopy.