European Journal of Biomedical and Pharmaceutical Sciences

IN VITRO AND IN VIVO ANTIOXIDANT ACTIVITY AND HEPATOPROTECTIVE POTENTIAL OF MORINGA PTERIGOSPERMAGAERTN. LEAF AGAINST CCL4 INDUCED HEPATOTOXICITY

Shah Anuttara*, Patil Darshana and Patil Avinash

ABSTRACT

This study aims to investigate the in vitro and in vivo antioxidant activity, hepatoprotective effect of Moringa pterigosperma Gaertn. in vitro antioxidant activity of Moringa pterigosperma Gaertn. leaves were investigated by using standard established assays. Mice with CCl4 induced liver injury were used to assess the antioxidant potential and hepatoprotective effect of aqueous slurry of Moringa pterigosperma Gaertn. leaves. Biochemical markers like SGOT, SGPT, ALP and Total Protein content were determined from blood serum. Antioxidant markers such as GSH, CAT, SOD and LPO were analyzed from liver homogenate using standard procedures. Moringa pterigosperma Gaertn. leaf extract exhibits good antioxidant potential in vitro. The in vivo results indicated that, treatment with aqueous slurry of Moringa pterigosperma Gaertn. leaf exhibited significant reduction in the levels of SGOT, SGPT and ALP in all groups. Out of various dosages, it was found that aqueous slurry of Moringa pterigosperma Gaertn. leaf administered at 750 mg / kg b.w. was most effective, as it maximally reduced LPO of liver with parallel increase in cellular antioxidants. Results were comparable with Silymarin, used as modern drug control. Findings from this study indicated hepatoprotective potential as well as significant antioxidant activity of Moringa pterigosperma Gaertn. leaf.

Keywords: Moringa, CCl4, hepatoprotective activity, antioxidant activity, in vitro, in vivo.