European Journal of Biomedical and Pharmaceutical Sciences

EVALUATION OF ANTI DIABETIC ACTIVITY OF SEEDS OF BLACK PEPPER IN STREPTOZOTOCIN INDUCED DIABETIC RATS

Parijatha Bandigari*, Ashma Mohammed, Sushma Arikilla, Bhaskar Chanduri, Shruthi Yemba and Krishna Mohan Chinnala

ABSTRACT

Diabetes mellitus is a metabolic disorder and emerging pandemic of the 21st century. Piperine, the chief alkaloid present in Piper nigrum (black pepper), has a wide array of uses in alternative and complementary therapies. The effect of piperine on blood glucose level was studied in streptozotocin induced diabetic rats in subacute study models. Piperine was isolated from the fruits of Piper nigrum crude extract. Diabetes was induced using streptozotocin in albino rats which were then randomly divided into 5 groups (n = 6). Drug intervention for subacute study consisted of once daily oral administration of distill water 10 mL/kg, metformin 150 mg/kg, piperine 20 and 50 mg/kg, in the control, standard, P20, and P50 groups respectively for 14 days,. Blood glucose levels were estimated before administration of drug and on day1, 7 and 14 in the subacute study respectively. A significant blood glucose lowering effect was seen with piperine at dose of 50 mg/kg on day 14 (p < 0.05) in the subacute study. In summary, we suggest that administration of piperine at the dose 50 mg/kg has statistically significant antihyperglycemic activity. Phytochemical investigation revealed the presence of alkaloids, as the major constituents in the plant Piper nigrum (black pepper). The results suggest that piperine (50mg/kg) showed antidiabetic activity in streptozotocin induced diabetic rats.

Keywords: Diabetes, Piperine, Piper nigrum, Streptozotocin and Metformin.