European Journal of Biomedical and Pharmaceutical Sciences

PREFORMULATION STUDIES AND PREPERATION OF RIFABUTIN LOADED SOLID LIPID NANOPARTICLES

Manisha Patel*, Gaurav K. Saraogi and Shailesh Sharma

ABSTRACT

Tuberculosis is a ubiquitous, highly contagious chronic granulamatous communicable bacterial infectious disease caused by Mycobacterium tuberculosis and other species of same genera. “Rifabutin” which is useful in the management of tuberculosis.Clinical management of tuberculosis posses serious problem because the efficacy of chemotherapy has been reduced which may be attributed to the degradation of drugs before reaching the target, the low level of cell permeability to drugs, or primary drug resistance. Other reason for the failure of chemotherapy may be the difficulty in achieving adequately high concentration at the infection site, inadequate penetration of drug into macrophages and low level in cells. These problems, which arise with conventional dosage forms of antitubercular drugs, may be overcome by designing and developing a site specific delivery of antitubercular drug using surface modified solid lipid nanoparticles. Hence in the present study, it was attempted to formulate Rifabutin in the form of solid lipid nanoparticle. Solid lipid nanoparticles of Rifabutin were obtained by adaption of lipid dispersion method. Preformulation studies were performed to check the compatibility of drug and excepient for the preparation of formulation by DSC and no interaction was found. Solubility study, partition coefficient determination, UV analysis, HPLC study, FTIR study were also performed. After the preformulation studies Rifabutin loaded solid lipid nanoparticles was also prepared. Hence it was concluded that solid lipid nanoparticle of Rifabutin could be formulated.

Keywords: Rifabutin, Tuberculosis, solid lipid nanoparticle, preformulation studies.