European Journal of Biomedical and Pharmaceutical Sciences

IN VITRO ANTIOXIDANT ACTIVITY OF MADHUCA INDICA L.

Anbukkarasi G. and Prasanna G.*

ABSTRACT

The present investigation was aimed to evaluate the phytochemicals and in vitro antioxidant activity of different parts of Madhuca indica. Qualitative phytochemicals analysis confirmed the presence of carbohydrates, tannins, flavonoids, quinones, glycosides, terpenoids, phlobatannins, alkaloids, phlobatannins, anthraquinones, coumarins, steroids and phytosteroids and absence of phenol, saponins and triterpenoids. In vitro antioxidant property was also carried out at five different concentrations (50, 100, 150, 200 and 250 μg/ml) of wood, bark and leaf by total antioxidant capacity, DPPH scavenging activity, hydrogen peroxide scavenging activity and reducing power assay. All the results were compared with standard ascorbic acid. Results showed that dose dependent antioxidant activity in all models. Among the three different parts, bark extract exhibited highest antioxidant activity followed by leaf and wood extract. Our result suggests that bark extract of Madhuca indica is a potential source of antioxidant which can be used to treat many free radical mediated diseases.

Keywords: Antioxidant, bark, leaf, Madhuca indica, phytochemicals, wood.