European Journal of Biomedical and Pharmaceutical Sciences

ANTI- MYCOBACTERIAL SCREENING METHODS FOR NEW COMPOUNDS IDENTIFICATION

Divya Murahari, Sireesha Tanniru, Lavanya Poonem Aleti Paramesh, Kamarapu Sudheer Kumar, Dr. Malathi Jojula* and Shravan Gunda

ABSTRACT

Tuberculosis (TB) is one of the oldest diseases and leading to cause a big number of deaths every year. The emergence of multidrug-resistant strains of Mycobacterium tuberculosis underscores the need of continuous developments on new and efficient methods to determine the drug susceptibility of clinical isolates of M.tuberculosis in the search for novel antimicrobial agents. Development of new drugs has become an immediate necessary to stop the prevalence of the Tuberculosis. As the present drugs were expressing complexity, toxicity to host and sensitivity towards the multidrug resistance TB, there is a need to develop or design newer drugs to treat TB in a short period and affordable. In the present study we tried to evaluate different methods for testing new synthetic compounds against Mycobacteria activity. Minimum inhibitory concentrations (MIC), Proportion method and MABA in liquid method were used to determine the potency of new antimicrobial agents according to RNTCP guidelines. A total of 101 compounds were screened and 28 compounds were identified in expressing their Anti-mycobacterial activity for both laboratory strain (H37Rv) and clinical isolate of MTB and MDR-TB, by using different analysis techniques (Spectrophotometer, Calorimeter, Turbidometer). In this study we screened newer synthetic compounds which may be helpful in the development of new TB drugs more rapidly to patients. We would like to conclude that, rapid method of detection of drug activity was noted by MABA in liquid media and we are able to differentiate within 5 days of time which was a rapid method for identification.

Keywords: Proportion based, MIC, Mycobacterium tuberculosis, MDR-TB.