European Journal of Biomedical and Pharmaceutical Sciences

EVALUATION OF THROMBOLYTIC AND MEMBRANE STABILIZING ACTIVITY OF ETHANOLIC NYMPHAEA NOUCHALI EXTRACT

*Rawnak Reza, Saikat Ranjan Paul, Md. Kamrul Islam Joy and Sayema Khanum

ABSTRACT

The present study is aimed to investigate in vitro thrombolytic and Membrane stabilizing activity of Nymphaea nouchali. Among the different fraction crude extract of leaf and stem. Nymphaea nouchali (Burm. f.) is commonly known as Blue lotus found in Sylhet, Bangladesh. DPPH radical scavenging activity. The thrombolytic activity of all extractives was evaluated by a method using streptokinase (SK) as standard substance. Commercially available lyophilized Altepase (Streptokinase) vial (Beacon pharmaceutical Ltd) of 15, 00, 000 I.U., was collected and 5 ml sterile distilled water was added and mixed properly. This suspension was used as a stock from which 100μl (30, 000 I.U) was used for in vitro thrombolysis., the ethanolic extract (EE) of stem inhibited 83.36% hemolysis of RBCs as compared to 83.52% produced by acetyl salicylic acid 10.0 mg/mL. Ethanolic extract of leaf and stem tested for clot lysis, crude ethanolic extract of stem proved its superiority maximum (54.829%)of clot lysis. Sterile distilled water as a negative control also showed negligible (13.357%) clot lysis. Standard Acetyl Salicylic Acid (ASA) or Aspirin was used as standard drug for comparison with different ethanolic extracts of leaves of N.nouchali. A buffer is an aqueous solution that has a highly stable pH. The buffer was prepared at pH 7 using monosodium phosphate and its conjugate base, disodium phosphate. The organic soluble materials of ethanol extract of the stems along with its various organic soluble partitionates were assessed for the membrane stabilizing activity as well as the thrombolytic activity of N. nouchali in the current study.

Keywords: Thrombolytic, Membrane stabilizing, blood cloat, Streptokinase, Aspirin.