European Journal of Biomedical and Pharmaceutical Sciences

HEPATO-NEPHROPROTECTIVE, HEMATOPOIETIC AND ANTI-SPERMATOGENIC EFFECT OF THE ETHANOLIC EXTRACT OF JATROPHA TANJORENSIS USING MALE ALBINO RATS

*Iroanya, Onyekachi Ogbonnaya, Egwuatu, Tochukwu Frank, Chukwudozie, Onyeka, Fadogba, Tolulope David and Adesanya Adenike

ABSTRACT

This study was conducted to ascertain the genotoxic, sperm boosting, hematopoietic, hepato-nephron-protective, properties of the ethanolic extract of Jatropha tanjorensis (EEJT) using male Wistar albino rats. The animals were randomly assigned to five groups of six animals each. Animals in group 1 were administered 10mgkg-1 water, groups 2-4 received 200, 400 and 800mgkg-1 EEJT respectively while groups 5 received 300mgkg-1 of Addyzoa for 64 days. Micronucleus assay, sperm cell count, hematological, biochemical, and antioxidant potentials were evaluated using standard methods. At 800mgkg-1, there was significant (p≤0.05) increase in the level of red blood cell (RBC), Haemoglobin (HGB) and Hematocrit (HCT) and significant (p≤0.05) decrease in ALP compared to Addyzoa. The extract dose dependently attenuated the concentrations of AST, ALP, total bilirubin, cholesterol, LDL, triglyceride and MDA compared to control and Addyzoa groups. Platelet, granulocytes, SOD, HDL, total protein RBC, HGB and HCT were dose dependently increased in the groups administered the extract compared to the control and Addyzoa groups. At 200mgkg-1, EEJT had the lowest number of binucleated and micronucleus cells. The highest number of abnormal sperm cells were seen in the group administered 800mgkg-1 of the extract. This experiment shows that EEJT dose-dependently improved erythropoietin promoting potential and hepatoprotective activity in the experimental animal. There was marked reduction in sperm count with sperm head abnormality at very high dosages. However, more research should be carried out to ascertain its mechanism of action and the dose to be administered over a long period.

Keywords: Genotoxic, Sperm Booster, Hematopoietic, Hepato-Nephron-Protective, Micronucleus Assay, Antioxidant.