European Journal of Biomedical and Pharmaceutical Sciences

TEST VIABILITY MICROENCAPSULATION LACTOBACILLUS ACIDOPHILUS USING ALGINATE-CHITOSAN POLYMER IN SIMULATED GASTRIC FLUID

Muhammad Junaidi*, Kasmirul Ramlan Sinaga and It Jamilah

ABSTRACT

Objective: Lactobacillus acidophilus is one of the strains of lactic acid bacteria that has been widely used as a probiotic. The ability of Lactobacillus acidophilus to grow in the digestive system can suppress the growth of enteric pathogenic bacteria and improve the balance of microflora in the digestive system so that it can be utilized to maintain a healthy body. Lactobacillus acidophilus bacteria exhibit short stationary phases and follow rapid cell viability loss, although stored at freezing temperatures. This study aims to determine the viability of bacterial microenkaosulasi to simulated gastric fluid (SGF). Methods: The microencapsulation of bacteria was performed by using a chitosan-alginate polymer, the method used was the extrusion method. Bacterial microencapsulation viability tests were performed by incubating microencapsulation in the simulated gastric fluid. Result: The microencapsulated Lactobacillus acidophilus ATCC 4356 cell population amounted to 3.9x107 colonies/ml of suspension, microencapsulated using sodium alginate-Chitosan polymer 2:0.1%; 2:0.2%; 2:0.3% with a cell density of 1.4x107; 1.6x107; 3.0x107 colonies/g can still be preserved Lactobacillus acidophilus cells incubated in SGF pH 1.5 for 120 minutes. Conclusion: Polymer sodium alginate-chitosan can maintain the cell number of the cell (Free cell) were still able to retain Lactobacillus acidophilus cells incubated in SGF pH 1.5 for 120 minutes.

Keywords: Lactobacillus Acidophilus, Lactic Acid Bacteria, Probiotic.