European Journal of Biomedical and Pharmaceutical Sciences

INTERACTION OF RED GRAPE AND NICOTINE WITH REFERENCE TO AGING IN THE MALE ALBINO RAT

Katika Khalindar Basha, Kotthapalli Govindu Reddy, Mala jayachandrudu, Mogarala Thejomoorthy, Katta Chennakesavulu, Kesi Reddy Sathyavelu Reddy, Koyya Chennaiah*

ABSTRACT

Red grape (Vitis vinifera.L ) presumably originates from Western Asia, from the area between the Caspian Sea region and Asia Minor, where also its cultivation seems to have begun . These archeological finds, up to this day the oldest documentation for the use of grape vine products in human culture, were dated to 3500-2900 B.C. Red grape is commercial juice products from Concord grapes have been applied in medical research studies, showing potential benefits against the diseases. Nicotine as most biologically active chemical in tobacco smoke. Nicotine has been reported to induce changes both in vivo and in vitro. Pathogen free, Wistar strain male albino rats were used in the present study. Rats were divided into 4 groups of six in each group i) Normal Control (NC) (Control rats received 0.9% saline) ; ii) Nicotine treated (Nt) (at a dose of 0.6 mg/ kg body weight by subcutaneous injection for a period of 2 months ); iii) Red Grape extract treated (RGEt) (red grape extract 50mg/kg body weight (after the standardization) via orogastric tube for a period of 2 months. ); IV)) Nicotine + Red Grape (Nt+RGEt) (Rats were received the nicotine with a dose as mentioned for Group II through subcutaneous injection and , red grape extract as mentioned for Group III via orogastric tube for a period of 2 months). The animals were sacrificed after last treatment by cervical dislocation and isolated the kidney tissue such as the activities of the levels of Total Cholesterol (TC), Triglycerides (TG), Phospholipids and Lipid peraxidation (LPO). In the present study, TC, TG and LPO was significantly increased in nicotine treated rats in the kidney tissue and the decreased was observed in the RGEt rats. In the combination treatment (Nt+RGEt) upregulation was observed in the kidney tissue. In case of phospholipids the decreased was observed in the nicotine treated rats in the kidney tissue. In the RGEt and Nt+RGEt the upregulation was observed in the kidney tissue. This investigation draws a conclusion stating that this much of red grape juice extracts to the old age as well as young age male subjects may be beneficial, especially for the nicotine subjects to improve the lipid metabolism efficiency and thereby to improve the health status and life span.

Keywords: Nicotine, Redgrapeextract, Total Cholesterol (TC), Triglycerides (TG), Phospholipids, Lipid peraxidation (LPO), kidney, male albino rat.