Abstract
UV SPECTROPHOTOMETRIC ASSAY METHOD FOR DETERMINATION OF CIPROFLOXACIN, TINIDAZOLE AND DICYCLOMINE IN COMBINED TABLET FORMULATION USING METHANOL: 0.1N HCl

Malathi Raghunath* and Amol Dhamne

ABSTRACT

The aim of the present study was to develop a simple, accurate, precise, reproducible and economical UV spectrophotometric method for estimation of Ciprofloxacin hydrochloride (CPX), Tinidazole (TNZ) and Dicyclomine hydrochloride (DIC) in combined tablet formulation. The method of absorbance corrected for interference was employed for quantitative estimation of DIC where as simultaneous equation method was used for estimation of CPX and TNZ. The UV absorbances were determined at 280 nm (λmax of CPX), 311 nm (λmax of TNZ) and 215 nm (λmax of DIC) respectively in methanol: 0.1N HCl (3:2). CPX and TNZ showed linearity at all the selected wavelengths, where as DIC showed zero absorbance at λmax of CPX and TNZ. The solutions of CPX, TNZ and DIC obeyed Beer’s law in the concentration range of 2-10 μg/ml, 2-20 μg/ml and 100-500 μg/ml respectively. The method was validated for parameters such as linearity, accuracy, precision, and limit of quantitation, limit of detection, ruggedness and robustness as per ICH guidelines. The proposed method showed good reproducibility and recovery and is thus recommended for routine analysis of fixed dose combination of these drugs in commercial tablet formulation.

Keywords: Ciprofloxacin, Tinidazole, Dicyclomine, Validation, Absorbance corrected for interference method, Simultaneous equation method.


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