European Journal of Biomedical and Pharmaceutical Sciences

SERUM FSH, LH, PROLACTIN AND TESTOSTERONE LEVELS AMONG SUDANESE PATIENTS WITH TESTICULAR AZOOSPERMIA

Eiman Ali Basheir* and Tarig Mohamed Fadl-Elmula

ABSTRACT

Background: Azoospermia is the medical condition of a man not having any measurable level of sperm in his semen. It is associated with sterility, but many forms are amenable to medical treatment. In humans, azoospermia affects about 1% of the male population and may be seen in up to 20% of male infertility situations. Male infertility can usually be diagnosed by testicular biopsy, spermiography and sometimes vasography. However, the first and the last of these methods are not always suitable, safe or acceptable to the patient, therefore looking for biochemical markers in serum and seminal fluid could help in the diagnosis of azoospermia. Methodology: This cross section study was done in 72 patient specimens, 41 azoospermic men (56.9%) and 31 normospermic men (43.1%). Men were attending Reproductive Health Care Center during June to August 2015 with complete medical and clinical histories. Patients’ age ranged from 25 to 59 years old. Men with azoospermia and the controls group were consented. Semen samples were analyzed manually as indicated by the WHO manual for semen analysis. Serum samples were analyzed for FSH, LH, Prolactin and Testosterone by ELISA using TOSOH analyzer. Results: Azoospermic patients have high levels of FSH and LH when comparing to the control group and when comparing to the normal range provided by the manufacture. Azoospermic patients have high level of prolactin when comparing to the control group but within the normal range provided by the manufacture, while have lower level of testosterone when comparing to the control group but within the normal range provided by the manufacture. Conclusion: Serum FSH, LH and Prolactin levels were statistically significant higher in patients with azoospermia than in normospermic Sudanese men (P-value<0.05). Serum testosterone level was statistically significant lower in patients with azoospermia than in normospermic Sudanese men (P-value <0.05). Azoospermic patients have high levels of FSH and LH, while normal levels of Testosterone and Prolactin were obtained when comparing to the normal range provided by the manufacture.

Keywords: Azoospermia, Follicular stimulating hormone (FSH), Luteinizing hormone (LH), prolactin and Testosterone.