European Journal of Biomedical and Pharmaceutical Sciences

AMARANTHUS SPINOSUS L. LEAVES - EXTRACTION, QUANTITATION & PURITY ESTIMATION OF GENOMIC DNA: EFFECT OF SEASON

Sudarshan Chettri, Ribek Thapa, Baibhav Karki, Tanaya Ghosh, Prasenjit Mitra and Prasanta Kumar Mitra*

ABSTRACT

Amaranthus spinosus L.(A. spinosus L.) is an important medicinal plant having several pharmacological properties. In the present investigation attempts have been made to isolate pure DNA from A. spinosus L. leaves with the objective that extraction of DNA from the plant will help the genotype analysis leading to scientific identification of the plant. As season has effect on synthesis of chemicals in the plant parts, attempts have also been made to study the seasonal effect on extraction of pure DNA from A. spinosus L. leaves. Leaves of A. spinosus L. were collected during autumn, winter, summer and rainy seasons, identified by experts and the leaves were processed for extraction, quantitation & purity estimation of genomic DNA by the conventional methods. Results showed that pure DNA in high amount was extracted from A. spinosus L. leaves during winter (December – February). It is concluded that A. spinosus L. leaves of winter season may be used to get high amount of pure DNA.

Keywords: Amaranthus spinosus L., DNA extraction, Purity estimation, Effect of season.