European Journal of Biomedical and Pharmaceutical Sciences

PHARMACOLOGICAL AND TOXICOLOGICAL EVALUATION OF LOPERAMIDE & NIACIN COMBINATION EITHER CANCER PROMOTER OR INHIBITOR IN DENA INDUCED LIVER CANCER USING WISTAR RATS

Pooja Negi, Kanchan Tripathi, *Kapil Kalra, Jyoti Maithani Kalra

ABSTRACT

Combination effect of Loperamide & Niacin in chemically induced hepatocarcinogenesis was assessed in Wistar rats. Intraperitoneal administration of chemical carcinogen diethyl nitrosamine (DENA) (200 mg/kg) in single dose elevated the levels of Serum Glutamate Oxaloacetate Transaminase (SGOT), Serum Glutamate Pyruvate Transaminase (SGPT), Serum Bilirubin (BIL), Total cholesterol (TC), Triglycerides (TG), Total leucocytes count (TLC), Alfa feto protein (AFP) and reduced Haemoglobin (Hb) and High density lipoproteins (HDL) level in tested animals. Histopathological examinations of the liver tissue showed marked carcinogenicity of the chemical carcinogen. Food and water intake, animal weights were also assessed. The animals exposed to DENA showed a significant decrease in the body weights and, there were no significant alterations found in the total leucocytes count (TLC). The elevated levels of serum SGOT, SGPT, BIL, TC, TG and AFP significantly decreased and increased HDL and Haemoglobin levels as compared to disease control group by administration of Loperamide (5 mg/kg) and Niacin ad libitum combination daily for 12 weeks P.O. Physiological and biochemical analysis showed the beneficial and synergic effects of Loperamide and Niacin combination in the animals exposed to DENA.

Keywords: Pharmacological evaluation, toxicological evaluation , cancer promoter, cancer inhibitor, wistar rats, DENA- N-diethyl nitrosamine, Loperamide, Niacin, SGOT- Serum glutamate oxaloacetate transaminase, SGPT - Serum glutamate pyruvate transaminase.