European Journal of Biomedical and Pharmaceutical Sciences

ISOLATION, PRODUCTION AND OPTIMIZATION OF ANTIMICROBIAL COMPOUND PRODUCED FROM SELECTED MARINE SOURCES

S. Kannan*, K. K. Senthil Kumar and M. Sivakumar

ABSTRACT

Currently, multiple antibiotic resistances in pathogenic bacteria is increasing, compromising the clinical treatment of growing number of infectious disease. There is an urgent need for new drugs effective against those antibiotic resistant pathogens and opportunistic pathogens. In the present work, samples like seawater, seashore sand and sediments from four different sampling sites, Vishakapatnam, Machilipatnam, Chennai and Kanyakumari. The marine bacterial species were isolated on Zobell Marine agar medium using spread plate technique. Among those isolates MB39 isolate showed maximum degree of inhibition against 6 strains of human pathogens like Staphylococcus aureus, Bacillus substilis, Escherichia coli, Klebsiella pneumonia, Salmonella typhi and Candida albicans compared to other isolates. The isolate was obtained from Vishakapatnam Sea Sediment sample (SS1), belonged to betaproteobacteria and phylogenetically related to Alcaligenes faecalis sp. was identified by according to the Bergey’s manual. For optimization the selected isolate MB39, grown by using Glucose as a carbon source showed good antimicrobial activity against the test pathogens. The degree of production was increased with increasing culture time with a maximum zone of inhibition obtained after 48 hours, after which decline in growth was observed at 72 hrs. Maximum zone of inhibition was observed at pH 9. For purification of the active compound solvent extraction was performed with five different organic solvents Chloroform, Methanol, Diethyl ether, Acetone and Ethyl acetate. Maximum activity was observed with ethyl acetate extract.

Keywords: Marine bacteria, Sea sediment, Antimicrobial activity, MB39, Betaproteobacteria.