EFFECT OF STACHYTRAPHETA INDICA ON HSP 60 IN CONTROLLING NEURODEGENERATION ON ROTENONE INDUCED PARKINSON’S DISEASE (PD) IN ZEBRA FISH MODELS
Dr.Muralidhar.S.Talkad*, Juganta Das, Pedenla Lama, Surabhi Chetia,
Dipanjan Dasgupta
ABSTRACT
Parkinson’s disease (PD) is quiet peculiar owing to its sudden and rapid occurrence. Several environmental toxins have been known to trigger PD. Therefore, such Neurotoxin-based models (particularly MPTP / Rotenone) have become preferred and important models in studying the degeneration of cholinergic and dopaminergic neurons. In this study we established Neuroprotective activity methanolic extract of Stachytarpheta indica on Rotenone induced Parkinson’s condition in Zebra fish. In addition oxidative stress enzyme GSH (0.189 ±0.0135 u/l) in liver of induced group, when compared to plant extract protection against oxidative stress on Zebra fish (0.327±0.0119u/l). The plant extract treated group showed the acetylcholine esterase enzyme activity (3.460 ±0.0208 μmols/min), it is closer to the Normal control group (2.940 ± 0.0025 μmols/min) as compared to the Parkinson’s induced group (4.882±0.0051 μmols/min). Since the inhibition or reduced activity of Acetylcholine esterase enzyme will curtail the progress of neurodegeneration in Parkinson’s and Alzehimer’s disease alike. The plant extract also shows acetyl choline inhibitory activity. The S.indica plant extract were shown Dopamine content 4.17% and reduce the increased activity of Acetyl choline esterase and re-establish the mitochondrial succinate dehydrogenase activity there by reducing neurodegeneration, it was confirmed from the expression of small Heat Shock Proteins was studied, and the S.indica treated group showed the expression of HSP 60 around 60 KDa, hence it is proven for the establishment of neuronal homeostasis.
Keywords: Neuroprotection, stachytarpheta indica, Parkinson´s disease (PD), Flavonoids, Rotenone, HSP60.
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