European Journal of Biomedical and Pharmaceutical Sciences

ROLE OF RED GRAPE EXTRACT AGAINST NICOTINE INDUCED CHANGES IN THE LUNG TISSUE OF MALE ALBINO RAT WITH REFERENCE TO AGING

M. Jayachandrudu, P. Muni Lakshmi, S. Kishore, K. Khalindar Basha and K. Chennaiah*

ABSTRACT

A grape (Vitis vinifera) is commercial juice product have been applied in medical research studies, showing potential benefits against the diseases. Nicotine, a major toxic component of cigarette smoking, has long been recognized to result in oxidative stress by inducing the generation of reactive oxygen species in the lung tissue. Wistar strain male albino rats were used in the present study, rats were divided into 4 groups of six in each group i) Normal Control (NC) (Control rats received 0.9% saline); ii) Nicotine treated (Nt) (at a dose of 0.6 mg/ kg body weight by subcutaneous injection for a period of 2 months); iii) Red Grape extract treated (RGEt) (red grape extract 25mg/kg body weight (after the standardization) via orogastric tube for a period of 2 months.); IV) Nicotine + Red Grape extract treatment (Nt+RGEt) as a mentioned in above Group II and Group III. The animals were sacrificed after 24 hrs after the last treatment by cervical dislocation and isolated the lung tissue. The levels of Succinate dehydrogenase (SDH), Isocitrate dehydrogenase (ICDH) and Malate dehydrogenase (MDH) were decreased in nicotine treated rats in the lung tissue and increases was observed in the combination treatment (Ni+RGEt) rats in both age groups. But at 25 mg/kg body weight were found more effective in both age groups. The Lactic dehydrogenase (LDH) activity was increased in all treated rats in the lung tissue then the control in both age groups, in nicotine treated rats in both age groups it is found more. This results stating that red grape extract treated rats are beneficial, especially for the nicotine subjects to improve the oxidative enzymes and thereby to improve the health status and life span.

Keywords: Nicotine, Red Grape extract, SDH, MDH, ICDH, LDH, Lung tissue and Male albino rat.