Apoorv Pankaj, Anu Chandra*, Tasleem Raza and Saliha Rizvi


CAD is one of the biggest cause of death around the world, with the number of people affected continually increasing. Increasing Incidence of CAD is a major concern not only in India but across the world. Genetic Polymorphism of many genes is associated with increased risk of various diseases in a particular ethnic group. This case control study was carried out to investigate the association of COL4A1 gene polymorphism with CAD. The study includes 25 angiographically proven CAD cases and 25 controls. Blood was collected in EDTA tube. Genomic DNA was extracted using phenol-chloroform extraction method (Sambrook J, 1989). Polymerase Chain Reaction(PCR) was done on extracted DNA. PCR product was digested with 5 U of restriction enzyme (Fermentas, UK). Statistical analysis was performed using SPSS 20.0 version. For SNP-1 rs605143 Frequency of COL4A1 AG, GG, AA genotype in CAD samples were 52%, 40%, 8% and in Control samples were 64%, 32%, 4% respectively. Frequencies of COL4A1 A and G alleles were 34% and 66% in CAD samples and 36% and 64% in control Samples. For SNP-2 rs 565470 Frequency of COL4A1 CT, CC, TT genotype in CAD samples were 60%, 12%, 28% and in Control samples were 52%, 16%, 32% respectively. Frequencies of COL4A1 C and T alleles were 42% and 58% in CAD samples and 42% and 58% in control Samples. Polymorphism of COL4A1 gene (rs605143, rs565470) was not associated with the risk of CAD (Statistically). For rs605143, frequency of A allele was significantly higher in Control subjects than in CAD patients which indicates that A allele of rs605143 is a protective factor for CAD while the frequency of G allele was significantly higher in CAD cases than controls suggesting it to be a risk factor for CAD. For rs565470, no significant difference was found between the allelic ratio of the case and the control samples.

Keywords: Coronary Artery Disease, COL4A1, Gene Polymorphism.

[Full Text Article]


Forgot Password  |  Register


Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Dr. Dhrubo Jyoti Sen

Download Article : Click here

News & Updation


    JUNE 2023 Issue has been successfully launched on 1 JUNE 2023.

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 5.467 to 6.044, due to high quality Publication at International Level.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.


  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.


UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS


Copyright From

Covering Letter

                        Author Instruction