European Journal of Biomedical and Pharmaceutical Sciences

IN VITRO ANTIPROLIFERATIVE ACTIVITY OF RETICULATACIN ON HUMAN PROSTATE CANCER CELL LINES (LNCAP AND PC-3)

Saritha Surapaneni*, T. Prakash1 and Dinesh Ghatamaneni

ABSTRACT

The prostate is a highly heterogeneous tissue. In addition to the luminal epithelial and tumor cells that have been the typical and traditional basis of in vitro models. Globally oncologists are looking towards compounds from natural origin that inhibits the growth of resistant tumor cells. We find that acetogenins are naturally occurring compounds that are chemically one of the least investigated families. For which anti-tumor activity has been documented within the literature. Reticulatacin as a potential anti-tumor activity is analyzed on the prostate cell lines, LNCaP and PC-3 cell lines was cultured and antiproliferative effect by MTT Method, Neutral red cytotoxicity, measurement of LDH (Lactate Dehydrogenase Assay) release, determinations of apoptosis by acridine orange (AO) and ethidium bromide (EB) double staining. Inhibition of protein denaturation, caspase levels by indirect ELISA and DNA fragmentation was performed. The results clearly depict increased fragmentation in PC3 upon compound treatment which can be attributed to the apoptotic activity of compounds. Results postulated that the Reticulatacin is more cytotoxicity and effective in inducing apoptosis. Double staining method (AO/Et-Br staining) for evaluating the cytotoxicity impact of Reticulatacin revealed the fact that the Reticulatacin is capable of inducing apoptosis.

Keywords: Reticulatacin, MTT assay, Lactate Dehydrogenase Assay, DNA fragmentation, caspase-3/7, Acridine orange, Ethidium bromide.