European Journal of Biomedical and Pharmaceutical Sciences

FORMULATION SPAN 40 NIOSOME OF AQUEOUS FRACTION MENIRAN (PHYLLANTHUS NIRURI L.)

Rise Desnita, Sri luliana*, Ronny martien and Arief Nurrochmad

ABSTRACT

Meniran (Phyllanthus niruri L.) is used for many conditions such as diabetes mellitus, hypertension, obesity and hyperlipidemia. The active constituens to which the antidiabetic activity of P. niruri L. has been attributed include flavonoids. Flavonoid glycosides have been the physical problem of delivering a drug across the membrane. The aim of the present study were total flavonoids content and to develop a niosomal formulation for aqueous fraction of P. niruri L. Methode in this study are analysis the total flavonoids content and characterization of the niosomes. The total flavonoids content from extract and aqueous fraction with rutin standard were 12.32±0.53 and 20.07±1.23 (RE/g) respectively. The niosomes preparation used a thin layer film hydration technique with solven hydration 0.5; 1; 2; 3 and 4% aqueous fraction of P. niruri L. Niosomes span 40 can be hydration in temperature 80oC for 20 minutes to concentration 0,5% and 1-3% for 50 minutes while 4% can’t released. The particle size of the optimal delivery system prepared with Span 40 100 μmol and cholesterol 20 μmol was about 5 μm.

Keywords: Aqueous fraction of P. niruri L., total flavonoids and niosome span 40.