CHEMOPROFILING OF HOMOEOPATHIC DRUG COFFEA CRUDA
Rakhi Mishra*, Manoj Kumar, Binit Dwivedi, B. S. Arya, Renu Arya, Anil Khurana and R. K. Manchanda
ABSTRACT
Chemoprofiling of homoeopathic drug/tincture (HT) represents a comprehensive approach for evaluation of quality, purity, safety and efficacy of HT. According to the phytoequivalence concept the full HT can be seen as the active compound, because the several constituents act together being responsible for its therapeutic effect. Coffea arabica (Coffee) is the healthiest beverages in the world is commonly called coffea cruda in homoeopathic system of medicine and frequently used in the treatment of insomnia, headaches, toothaches and labor pains. The main active constituents of Coffea cruda are caffeine, trigonelline, chlorogenic acid, quinic acid, tannic acid, tannin, fixed oil and proteins. Present study reveals the moisture content (3.683 %), Total Ash (2.66 %), Alcohol (6.48 %), Water extractive values (21.69 %), total solids (0.48 %), Wt/ml (0.82 g) and Alcohol content (90.0 %). In UV spectroscopy λmax. at 280 nm shows presence of caffeine in HT. HPTLC analysis of in house HT, caffeine standard and three market sample were performed by using chloroform methanol (9:1, v/v) as mobile phase. Under UV light (254 nm), spot of caffeine was observed at Rf. 0.56 in above samples. However excess amount of caffeine content found in In-house HT rather than the market samples.
Keywords: Coffea arabica, Homoeopathic drug, Chemoprofiling, H.P.T.L.C fingerprint.
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