European Journal of Biomedical and Pharmaceutical Sciences

ANGIOTENSIN-I-CONVERTING ENZYME (ACE) INHIBITORY ACTIVITY AND NITRIC ACID PRODUCTION OF PHENOLIC COMPOUND EXTRACTED FROM Turbinaria conoides

Edward Joseph Gallyot, Arumuganainar Suresh*, Khushboo Kumari and Natarajan Velmurugan

ABSTRACT

Angiotensin-I-converting enzyme (ACE) plays a vital role in regulation of blood pressure by converting angiotensin I to angiotensin II, a potent vasoconstrictor, whereas nitric acid (NO) is a vasodilator. Hence inhibition of ACE and induced NO production is considered to be a positive therapeutic approach for hypertension related ailment. Marine algae phenolic compounds possess ACE inhibitory activity, therefore, this study was carried to examine the ACE inhibitory and NO production of phenolic compounds from the marine brown seaweed Turbinaria conoides. The algal samples were collected from Mandapam, Tamil Nadu. Different solvent (ethanol, ethyl acetate) extracts were prepared and quantified for phenolic content and examined for ACE inhibition and NO production. Ethanol extract 83% phenolic compounds and shown strongest ACE inhibitory activity with an IC50 value of 0.81 mg/mL followed by ethyl acetate extract (73% and 1.24 mg/mL IC50 value). The phenolic compound had an inducible effect on the NO production (36%) in MCF-7 cell line without having cytotoxic effect (94% viability) when up to 100 ng of the extract applied. GS-MS data revealed the presence of four major compounds which validates above biological observations. This study found that T. conoides could be a potential source of phenolic compounds with ACE inhibitory activity, which may be exploited in the future to prevent hypersensitive related disease.

Keywords: Brown algae; Ethanol extract, Hypertension, Seaweed, Vasodilator.