European Journal of Biomedical and Pharmaceutical Sciences

STUDIES ON HIGH FREQUENCY MULTIPLE SHOOT INDUCTION OF SOLANUM SURATTENSE BURM F FROM FLORAL BUD EXPLANTS

Rajendra Prasad B. Prameela K., Waghire Bhausaheb and Ugandhar T.*

ABSTRACT

An efficient and reproducible protocol has been developed for in vitro propagation from floral bud in Solanum surattense on MS medium fortified with various concentrations of cytokinins such as BAP and Kn individually and also in combination with auxins IAA(0.5mg/L)+BAP/Kn (1.0-8.0mg/L) for multiple shoot induction. High multiple shoot buds/explnt (19.0 ± 0.35) proliferation was observed at IAA (0.5mg/L) and BAP (3.0mg/L) from the floral bud explants within four weeks of culture was attained. Individual shoots were aseptically excised and sub cultured in the same media for shoot elongation. The elongated shoots were transferred to (IBA) (1.0–5-0 μM) for root induction. Rooting was observed within two weeks of culture. Rooted plantlets were successfully hardened under culture conditions and subsequently established in the field conditions. The recorded survival rate of the plants was 96%. Plants looked healthy with no visually detectable phenotypic variations.

Keywords: In Vitro Proliferation, High frequency, floral bud explants, Solanum surattense. Burm.F.