European Journal of Biomedical and Pharmaceutical Sciences

EVALUATION OF PREDOMINANT COMMERCIAL VACCINES IN PROTECTION FROM AVIAN INFLUENZA DISEASE H5N1 IN BROILER CHICKENS

Disouky Mohamed Mourad*

ABSTRACT

With regard of economic importance of H5N1 avian influenza disease, some vaccines were evaluated against avian influenza experimental infection. 5 groups of one day old chick each group 30 chicks, group 1 non vaccinated non challenged; group 2 vaccinated with Vectormune then challenged; group 3 vaccinated with inactivated H5N2 then challenged, group 4 vaccinated with Inactivated H5N1 then challenged and group 5 non vaccinated challenged. serum samples were collected weekly and the HI titers were determined. The highest HI titers and protection percent was recorded in group vaccinated with Vectormune followed by group vaccinated with inactivated H5N1 then group vaccinated with inactivated H5N2. typical clinical signs and post mortem lesions of highly pathogenic avian influenza virus were observed in groups 3, 4 and 5 but Vectormune vaccinated group showed certain mortalities without obvious clinical signs. Tracheal shedding were detected by RT-PCR, Vectormune vaccinated group recorded the lowest shedding followed by H5N2 then H5N1 vaccinated group.

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