European Journal of Biomedical and Pharmaceutical Sciences

IMMUNOHISTOCHEMICAL EXPRESSION OF VEGF IN RENAL CELL CARCINOMA AMONG SUDANESE PATIENTS

Hayat Ali Ahmed Fadul* and Dr. Ageeb Mohammed Hassan

ABSTRACT

Background: Renal cell carcinoma (RCC) is the most common adult renal epithelial cancer in the United States and the ninth most common malignancy in Europe in 2008 until recently. Renal cell carcinoma continues to be a difficult malignancy to treat because of its ability to spread asymptomatically and its inherent resistance to conventional chemotherapy. Objective: To evaluate immunohistochemical expression of vascular endothelial growth factor in renal cell carcinoma in our population and correlate its expression with different variables such as age and grade, also determination the specificity of vascular endothelial growth factor in renal cell carcinoma by using immune-histochemical technique. Materials and methods: this descriptive cross sectiona studyl for analyzing data from 40 formalin-fixed of paraffin-embedded tissues. These sections from all cases were subjected to stain with haematoxylin and eosin (H&E) for histopathological examination and immunohistochemical technique for VEGF. Results: The results show that 22 of 40 (55%) with grade 1 and 3 each one had 11(27.5%) frequencies and 12 (30%) frequencies with grade 2 while grade 4 had 6 (15%) frequencies, also we found that 32 (80%) diagnosed with clear cell renal cell carcinoma ccRCC, 2 (5%) chromophope, 5 (12.5%) papillary and 1(2.5%) was collecting duct, the expression of vascular endothelial growth factor marker in renal cell carcinoma were 29 (72.5%) out of 40 samples, the expression rate of ccRCC, papillary and chromophope were 24 (60%), 3 (7.5%) and 2 (5%) respectively for vascular endothelial growth factor marker with significant (.211), the expression rate of vascular endothelial growth factor marker in grade 1,2,3 and 4 were 3 (7.5%), 9 (22.5%), 11 (27.5%) and 6 (15%) respectively with significant (0.000). Conclusion: In this study we conclude that the expression of VEGF marker in grade 1 and 2 were 30% while in grade 3 and 4 was 70% so it’s had a higher microvessele density than those without or low expression. Also we show that the specifity of vascular endothelial growth factor marker were 72.5% and must be used in investigations as prognostic value.

Keywords: Vascular endothelial growth factor, Immunohistochemistry, Histopathology.