European Journal of Biomedical and Pharmaceutical Sciences

CHITOSAN ANCHORED NANOPARTICLES OF METHOTREXATE FOR THE MANAGEMENT OF BREAST CANCER .PREPARATION AND EVALUATION :PART-II

Rajkumari Thagele and Mohan Lal Kori*

ABSTRACT

Objective: This study was aimed at examining an effect of Methotrexate loaded chitosan nanoparticles on growth and proliferation of breast cancer cells. Background: Breast cancer is one of the most common cancers and the second leading cause of cancer death among women worldwide. MTX, is an analogue of folic acid, which exhibits not only a targeting role as folic acid but also a therapeutic effect to many types of cancer cells that overexpress folate receptors on their surfaces. We hypothesize that Methotrexate loaded chitosan nanoparticles can showed better result from this anticancer drug. Methods: Breast cancer cell lines, MCF-7, were treated with developed Methotrexate loaded chitosan nanoparticles at 24, 48 or 72 hours for MCF-7. We used the MTT assay and lactate dehydrogenase leakage (LDH) assay to evaluate cell viability and cytotoxicity, respectively. Conclusion: We have demonstrated that Methotrexate loaded chitosan nanoparticles were internalized well in breast cancer cells in vitro, suggesting their suitability in breast cancer treatment. Preferential uptake of nanoparticles rather than the free drug by MCF-7 cells causes the cells to be more viable to the free drug.

Keywords: Breast cancer, Methotrexate, Chitosan, MTT.