Abstract
ANALYSIS OF DOFETILIDE IN BULK AND FORMULATIONS BY VALIDATED SPECTROFLUORIMETRIC METHOD

Sangeetha R. K.*, Sangeetha V., Sree Varshini S., Sowmiya S. and Ravi T. K.

ABSTRACT

The selected drug dofetilide do not have any native fluorescence. The structure contains tertiary amide with a oxide group in the aliphatic chain attached with the benzene ring. Hence an attempt was made to convert the compound into a fluorescence emitting moiety by treating with chromotropic acid in the presence of sulphuric acid at a temperature of 100ᵒC. The drug was treated with 0.25 ml of sulphuric acid and 1 ml of 0.01% chromotropic acid and placed on a thermostat maintained at a temperature of 100ᵒC for 15 minutes. The chemistry of the reaction involves the breakage of the aliphatic chain oxide group. The second step involves the attachment of the chromotropic acid resulting in the formation of fluorescence compound. The fluorescence product formed was measured in the UV Visible region. The excitation was found at 359 nm and emission at 707 nm. The linearity was found between 0.25 to 1.5 mcg/ml with a correlation coefficient of > 0.99. The percentage RSD value for the precision studies and accuracy was less than 2. The method developed was validated according to the ICH guidelines and can be applied for the routine analysis of dofetilide in bulk drugs and formulations.

Keywords: .


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Tangudu Nagabhusana Rao

Download Article : Click here

News & Updation

  • NEW AUGUST ISSUE PUBLISHED

    AUGUST 2020 Issue has been successfully launched on 1 August 2020.

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 5.467 to 6.044, due to high quality Publication at International Level.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT