Abstract
A COMPARATIVE ANALYSIS OF THE ANTIBACTERIAL ACTIVITY OF BERBERIS ARISTATA AND BERBERIS ASIATICA ON GASTROINTESTINAL PATHOGENS

Shraddha K. C.*, Shobha Amagain, Shyaron Poudel and Anup Basnet

ABSTRACT

Objective: The study had been envisioned to assess the antibacterial activity of the different parts viz. root, bark, stem, leaf and fruit of Berberis aristata and Berberis asiatica with a view to compare the potentiality of these respective plants as an antimicrobial agent. Methods: The study involved the identification, collection, extraction and antibacterial analysis of the respective extract. The crude extract was prepared by Solvent Extraction method to make a final concentration of 100mg/ml and analysed using Agar well diffusion method against 6 gastrointestinal pathogens: Bacillus subtilis (ATCC 23857), Enterococcus faecalis (ATCC 29212), Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Salmonella Typhi (ATCC 27870). Results: The extract was overall more effective against gram positive organisms. The root and bark extract of both plants presented the maximum inhibitory action against all test organisms in a comparable range. The root extract was most effective against B. subtilis with respective inhibition size of 24mm and 22mm for B. aristata and B. asiatica. The bark extract showed the highest activity against S. aureus for both plants. Stem extract of B. aristata and B. asiatica inhibited 5 and 3 bacterial strains repectively. The leaf and fruit extract of both the plants exhibited the least activity. Conclusion: The present study reflected the potentiality of B. asiatica as a substitute to B. aristata as an antimicrobial agent and emphasizes the need to screen for the overall study of the plant so as to utilize them for therapeutic purposes.

Keywords: Berberis aristata; Berberis asiatica; Agar well diffusion; Gastrointestinal pathogens; Antibacterial activity; Zone of Inhibition.


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Tangudu Nagabhusana Rao

Download Article : Click here

News & Updation

  • NEW AUGUST ISSUE PUBLISHED

    AUGUST 2020 Issue has been successfully launched on 1 August 2020.

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 5.467 to 6.044, due to high quality Publication at International Level.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT